- Ready-to-use in widefield fluorescence, confocal microscopy, and immunofluorescence assays. No autofluorescence, has bright fluorescence signals. In dropper bottle for exact dispensing into channels or open wells.
Mounting medium with DAPI optimized for fluorescence microscopy and ibidi µ-Slides and µ-Dishes - 15 ml
Applications:
- Immunofluorescence assays with nuclear DAPI counterstaining
- Widefield fluorescence and confocal microscopy
- Short-term and long-term storage of stained slides and dishes
Technical Features:
- Specially designed for all ibidi µ-Slides, µ-Dishes, and µ-Plates
- Contains DAPI for nuclear counterstaining, also available without DAPI
- Contains antioxidant agent (DABCO) to preserve fluorochromes
- Provides optimal pH for detecting fluorescence signals
- Non-drying, non-hardening, and volume-stable
- Low autofluorescence for reduced background
Specifications:
Appearance | Liquid, optically clear |
pH | 8.5–9.0 |
Refractive index nD | 1.42–1.44 |
Staining specificity | DNA/RNA (nucleus) |
DAPI-DNA (Exmax / Emmax) | 358 / 461 nm |
Storage of mounted samples | In the dark at 2–8°C |
Product storage | In the dark at 2–8°C |
Low Bleaching of Fluorochromes in Stained Samples
The ibidi Mounting Medium with DAPI contains the anti-bleaching reagent 1,4–diazabicyclo[2.2.2]octane (DABCO). This antifade agent drastically reduces photobleaching of fluorochromes in immunofluorescence microscopy. This preserves a strong fluorescence signal in the sample.