Analysis of the importance for the development of new therapies and engineered tissues. Selected publications with a discussion of studies conducted with the use of rat collagen type I obtained from the tail of a rat.
Guide
Collagen Type I, Rat Tail 5 mg/ml - 1x 5ml
Code: 50201
- A high-quality rat tail collagen type I hydrogel for cell culture applications (e.g., 3D gels, scaffolds, and coating)
A high-quality rat tail collagen type I hydrogel for cell culture applications (e.g., 3D gels, scaffolds, and coating)
Concentration: 5 mg/ml
Quantity: 5 ml
Technical Features:
- High-quality purified protein from rat tendons
- Highly standardized manufacturing process with stringent quality control
- Mild manufacturing process for highest levels of nativity
- Detailed protocol provided for easy handling
- Shipping on cool packs and storage at –20°C for well-defined quality and reproducibility
- Available in 2 concentrations: 5 mg/ml for standard applications and a high-concentrated solution with 10 mg/ml for special applications
- Highest level of natural cross-linking on the market (highest quality grade)
- High viscosity: special pipettes for highly viscous solutions are recommended
- Low autofluorescence
- Provides in vivo-like extracellular matrix (ECM) structures
- Non-pepsinized, native collagen for modelling biological ECM in gel matrices
- Fast polymerization facilitates optimal cell distribution in 3D gels
Applications:
- 3D cell culture in in vivo-like matrices
- Live cell imaging in a 3D environment
- Creating a 3D scaffold for organoids and spheroids
- Chemotaxis assays in 3D collagen gels using the µ-Slide Chemotaxis
- Cell migration assays of suspension cells (e.g., leukocytes)
- Coating of cell culture vessels with a thin collagen layer to promote cell attachment
- Cell differentiation studies in a 2D versus 3D environment
- Highly suited for fluorescence microscopy due to low autofluorescence
Specifications:
- Source material: Rat tail tendon
- Appearance: Optically clear viscous liquid
- Extraction: Acid, non-pepsinized
- Purity: > 90% by SDS PAGE
- Sterility: Sterile, for cell culture
- Quality: Tested and found negative for DNA, bacteria, fungi, and mycoplasma
- Growth factors: None
- Formulation: Supplied in 17.5 mM acetic acid (0.1%)
- pH: 3.8
- Functional control: 3D gelling and 2D coating test in cell culture
- Storage: –20°C, avoid multiple freeze and thaw cycles
A product with special transport requirements. Transportation is carried out under temperature control using dry ice.
Brand
Symbol
50201
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